Mammalian and Bacterial Cell Culture and CRISPR GENE Editing
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About the Course
Introduction to Stem Cell Human Cell & Cancer Cell Culture
Basic Cell Culture Techniques
Media Preparation and Sterility testing
Sub Culturing of Cell Culture & Passaging and Maintenance of Cell Line
Cryopreservation and Cryo-revival
Staining of Cells
RNA Isolation from Mammalian Cell
DNA Isolation from Mammalian Cell
Introduction to GLP, QC, QA
Quantification of Nucleic Acid
Isolation of Stem Cells from Peripheral Blood
Preparation of PBS Buffer and Antibiotic Stock Solution
Cell Counting and Cell Viability by Hemocytometer
Total Protein Extraction
CRISPR Gene Editing
Agarose Gel Electrophoresis
Estimation of Protein
Ouchterlony Double Diffusion
Sputum, Stool, Urine & Serum Test
Isolation of single microbe pure colonies
Maintaining Bacterial Cell Culture with cryopreservation
Bacterial Identification by Staining
Catalase, Oxidase, Urease, Dextrose Tests
MR, VP, Indole, Mannitol, Citrate Tests