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Mammalian and Bacterial Cell Culture and CRISPR GENE Editing

Duration

60 Days

Fee Details​​

20000

How to Register

Click on the registration link and enter your details.

Then complete your enrollment by clicking on the enrollment link and paying 3000 (use Gpay option).

Then mail your copy of Aadhar, Last Marksheet, College/University/Job ID & the payment screenshot to informaticsbose@gmail.com. Mention the subject of mail as INTERNSHIP and mention the starting date, topic you chose and duration of the course you want to persue in the mail.

We will return mail you with the registration and enrollment details ASAP.

About the Course

Introduction to Stem Cell Human Cell & Cancer Cell Culture
Basic Cell Culture Techniques
Media Preparation and Sterility testing
Sub Culturing of Cell Culture & Passaging and Maintenance of Cell Line
Cryopreservation and Cryo-revival
Staining of Cells
RNA Isolation from Mammalian Cell
DNA Isolation from Mammalian Cell
Introduction to GLP, QC, QA
Quantification of Nucleic Acid
Isolation of Stem Cells from Peripheral Blood
Preparation of PBS Buffer and Antibiotic Stock Solution
Cell Counting and Cell Viability by Hemocytometer
MTT Assay
Total Protein Extraction
SDS Page
RT-PCR
CRISPR Gene Editing
Agarose Gel Electrophoresis
PCR
Estimation of Protein
Ouchterlony Double Diffusion
Sputum, Stool, Urine & Serum Test
Isolation of single microbe pure colonies
Maintaining Bacterial Cell Culture with cryopreservation
Bacterial Identification by Staining
Catalase, Oxidase, Urease, Dextrose Tests
MR, VP, Indole, Mannitol, Citrate Tests

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