Cancer Immunology with CRISPR CAS-9

Duration

30 Days

How to Register

Click on the registration link and enter your details. Then mail your copy of Aadhar, Last Marksheet, & College/University/Job ID to informaticsbose@gmail.com. Mention the subject of mail as INTERNSHIP with the starting date, topic you chose and duration/version of the course you want to persue in the mail. Once we approve your request after reviewing your application, then complete your enrollment by clicking on the enrollment link and paying the fees (use Gpay or UPI).

We will return mail you with the registration and enrollment details along with the invoice ASAP.

About the Course

Introduction to GLP, QC, QA
MIC & IC-50 against Sepsis
CRISPR Gene Editing
Preparation of PBS Buffer
Preparation of Antibiotic Stock Solution
Preparation of Cryomix for Mammalian Cell
Protein Extraction from Animal Tissue
Antigen-based agglutination assay
Preparation of blood samples for membrane stabilisation assays (Anti-INF Assay)
Hypotonicity-induced hemolysis (Anti-INF Assay)
Membrane Lysis Assay (Anti-INF Assay)
Heat-Induced Hemolysis (Anti-INF Assay)
Protein Denaturation Assay (Anti-INF Assay)
Proteinase Inhibitory Activity (Anti-INF Assay)
Isolation of mRNA
Isolation of mi-RNA
Ouchterlony Double Diffusion
PCR
Agarose Gel Electrophoresis
COOMB’S Assay
SDS Page
ELISA
Isolation of Stem Cells from Peripheral Blood
Cell Counting by Hemocytometer
DNA Isolation from Mammalian Cells
RNA Isolation from Mammalian Cells
Protein Isolation from Mammalian Cells
Quantification of Nucleic Acid
Estimation of Protein
Cryopreservation and Cryo-Revival
Culturing of Cancer Cells
Passaging and Maintenance of Cell Lines
MTT Assay
Apoptosis
ROS Assay
Western Blot
Animal Handling
Genotyping
Phase/Fluorescence Microscopy
H&E Cell Staining
RT-PCR

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